The molecular biology revolution has required the development of new chromatographic techniques and the optimization of original techniques to give reasonable quantities of protein at high resolutions. The aim of this volume is to provide the necessary information in most experimental situations to enable rapid and effective purification. The first four chapters deal with the instrumental aspects of high resolution chromatography starting with the initial clean up steps prior to separation in chapter 1. Chapter 2 deals with microscale techniques, then chapter 3 describes the detector technologies that can determine information about the separated molecules. The final chapter in this section cover capillary electrophoresis and its associated techniques. The remaining chapters cover a range of chromatographic procedures based on the interaction of a specific ligand with its target protein or other macromolecule. Some chapters cover non-specific interactions using peptides, inhibitors, and antibodies as the affinity ligand while others focus on specific groups of molecules : oligosaccharides and glycosylated proteins, nucleotide-binding proteins, proteins binding free and chelated metal ions, and DNA binding proteins.
Get on board for the Emotional Roller Coaster Series. Your first ride is the Katapult Roller Coaster. You will get unique, proven, easy solutions for all the ups and downs of launching your kids. Catapult yourself onto a fun ride with brain exercises that will amuse you while changing your life. Many of these experiential psychological techniques will not only change your thinking, but will spin other areas of your life (you do have a life outside of your kids, don't you?) in an even more successful direction. You will be inspired and empowered to: *blast through strategies for the years before your child launches *propel your relationship with your teen from good to Wow! *change your mental pictures, thus changing your emotions and behaviors *convict yourself of good parenting *turn sadness, worry or guilt into joy, confidence and pride ****BONUS Worksheets included to guarantee you will make changes and reap the rewards All of the Emotional Roller Coaster Series volumes are fun, short, easy to read books that give you the solutions you need without taking much of your time. Whether your child just left home, left years ago, won't leave for a few more years, or you are just hoping your child will leave home soon, you will enjoy a wealth of benefits from reading this book.
This book, designed as a handbook, provides a systematic treatment of analytical solutions describing groundwater flow during aquifer tests. The book integrates the majority of known solutions from well hydraulics and subsurface flow theory, starting with pioneering work from the early 20th century up to the most recent publications in scientific journals. The book includes about 300 transient solutions covering a wide range of aquifer test scenarios and hydrogeological conditions. All the solutions have been thoroughly tested and implemented in the multifunctional ANSDIMAT software. The book comprises three parts and is supplemented by appendices.
The first part of the book is dedicated to basic analytical relationships referring to pumping tests with constant discharge rate. Conceptual models describe confined, unconfined, confined-unconfined, inhomogeneous, and fracture-porous aquifers, as well as leaky aquifers and multi-layer aquifer systems. Complicating factors such as flow boundaries, aquifer anisotropy, non-uniform aquifer thickness, partial well penetration, wellbore storage and skin, the effect of capillary forces are also considered.
The second part focuses on complex pumping test settings and well system configurations. Analytical solutions are presented for pumping from a horizontal or inclined well, constant-head tests, multi-well variable-discharge tests, simultaneous pumping from adjacent aquifers and dipole flow tests. Detailed descriptions are given for slug and recovery tests.
The third part of the book contains algorithms for evaluating hydraulic characteristics using analytical and graphical methods, and is supplemented by the ANSDIMAT tool. This software includes solutions for some practical engineering-hydrogeological problems, in particular, the assessment of aquifer characteristics by data on groundwater level monitoring and the evaluation of water inflow into open pits.
The book is supplemented with appendices in which hydrogeologists can find a vast body of useful information including mathematical descriptions of the majority of analytical functions used in the book, their plots and possible approximations.
The book is useful for hydrogeologists (students, engineers and researchers) engaged in groundwater flow studies, aquifer test analysis, environmental geologists and civil engineers. Experts in water flow numerical modeling and programmers developing software for aquifer tests will find valuable information in this book, which can also be used for educational and research purposes.
Recent large-scale corporate collapses, such as Lehman Brothers, Enron, Worldcom, and Parmalat, highlight the implosion of traditional models of fraud prevention. By focusing on risk factors at the micro level, they have failed to take into account the broader context in which external auditors operate as well as the crucial importance of such factors as corruption, organizational culture, corporate social responsibility, ethical values, governance, ineffective regulation, and a lack of transparency. Corporate Fraud and Corruption engages readers by showing how evidence-based, multi-level micro and macro analysis of fraud risk and protective factors inform effective fraud prevention, in turn minimizing financial catastrophes. Krambia-Kapardis focuses on her own empirical research into the aetiology of fraud to showcase a holistic approach to fraud prevention. This book also features major case studies from the United States, the United Kingdom, and Australia.
Garlic may be a little plant, but it delivers big results. Not only does it put flavor and zest in any dish it's added to, but it's packed with 200 chemical compounds, vitamins, and minerals that work together to make it a nutritional wonder. Add to that its antibacterial, antiviral, antioxidant, and antifungal properties, and you're looking at a food truly deserving of the "super food" title. Whether you're a garlic connoisseur, a weekend gardener, a curious cook, or a health-conscious baby boomer, Garlic Solutions will help you make the most of your homegrown or store-bought garlic. Here's a taste of what's inside: * From fresh to freeze-dried, from powdered to pills, an in-depth look at the types of garlic available and the best way to use each. * Which type of garlic a noted cardiologist says you should take daily alongside your multivitamin. * How to grow your own garlic, no matter where you live. It's easier than you think. * How to create a "garlic barrel," a fun way to include your kids in garlic cultivation. * Tips, tricks, and hints to make cooking with garlic a hassle-free and flavor-rich experience. * The easy way to roast garlic and a multitude of ways to use it. * Simple recipes for garlic bread, butter, and more. * The three components of cardiovascular health and how garlic benefits them all. * Garlic's newest scientific frontier: fighting cancer. * An antibacterial and antiviral drink to keep you healthy during cold and flu season. * A unique way to remove garlic odor from your hands. All you need is a spoon. * Folk remedies for maladies A through Z from all over the world. * Unusual uses for garlic. Hint: one involves marshmallows and fish. * Garlic trivia, fun facts, and smile-inducing quotes, from Shakespeare to Dr. Seuss. Garlic Solutions is all this and more! Start reading today. Start living better tomorrow.
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